About the “Multisense Chip” project

The aim of the proposed project is the development of a portable analytical instrument to carry out the detection and identification of biological pathogens on the molecular and immunological levels.


This system will be based on a portable instrument plus a lab-on-a-chip as the only comsumable. It will not only be able to carry out the detection reactions, namely the PCR and immunoassays, but also the sample enrichment and the extraction of the target molecules from the sample via the implementation of the complete sample preparation on the lab-on-a-chip device.


Detection will be achieved via optical, electro-optical or electrochemical means. All functions following the sample uptake and transfer are integrated on chip. This is to avoid all manual handling steps as well as the need for specially equipped laboratories and trained personnel. This means a “sample in, result out” system will be at hand for the confident & required detection of multiple pathogens a system that, directly highlighting the analytical results on molecular and immunological levels.

Applications

Objectives

  1. 1.Nano- and microfabrication technologies and 3D nanostructures


  1. enable miniaturization of the system

  2. allow for detection at the point of interest

Enabling technologies

  1. 2.Sensor technology based on electro-optical (electrochemilumines-cent) and electrochemical sensors


  1. novel fabrication strategies allow direct integration on plastic chips

  2. detection on the immunologial and molecular level

  1. 3.Biosensors and protocols


  1. high specificity and sensitivity

  2. after protocol validation with lab-based instruments, all protocols will be transferred to the integrated chip platform

  1. 4.Scientific instrumentation


  1. instrument should control the fluidic, thermal, electronic and optical behaviour

  2. robustness and portability are also targeted

Samples that will be handled by the proposed system:


                        1. Air


                       2. Solid samples


  1. powder

  2. contamination on the surface collected with a swab


                        3. Liquid samples


  1.  beverages

  2. water



User cases for that the proposed system will be applied:


                        1. One-time use of the lab-on-a-chip device


  1. reagent storage integrated in the consumable


                        2. Permanent monitoring


  1. monitoring in public or critical environments

User scenarios

After the first successful stage of concept, the identification of the completing B-agent spectrum will be transferred to the platform:


  1. Yersinis pestis

  2. Francisella tularensis

  3. Burkholderia mallei

  4. Burkholderia pseudomallei

  5. Brucella melitensis

  6. Brucella abortis

  7. Coxiella burnetii

  8. Bacillus anthracis

Targets

The chip based process flow depicted below includes the collection of the sample, its transfer to the chip, the biological reactions on chip and the two detection reactions. In order to avoid contamination the entire waste storage is also organized on chip.

Process Flow

Off-chip

On-chip

Air

Swab

Liquid


Sample preparation

Immunological reaction

Molecular reaction




Sample transfer

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5. Technology Integration


  1. multidisciplinary network merging engineering science with the biological and diagnostic fields, advanced sensor technologies and scientific instrumentation